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$Mouse OPC growth is accelerated when cultured in medium supplemented with B104CM and <t>FGF-2</t> and maintained under physiological oxygen conditions. The enriched OPC fraction from neonatal C57Blk/6 mouse mixed glial cell cultures was plated onto fibronectin coated tissue culture plates at 3x10 4 cells/cm 2 and cultured in PDGF-AA+FGF-2 or B104CM + FGF-2 and propagated in 20% oxygen (A, B) or 2% oxygen (A1, B1) for 5 days. The number of cells obtained when grown in B104CM + FGF2 in either 2% or 20% oxygen over 4 serial passages across 35 days is depicted (C). The enriched OPC fractions from mixed mouse glial cell cultures plated onto fibronectin (D) and from mixed rat glial cell cultures plated onto poly-d-lysine (E) and cultured in B104CM + FGF2 in 20% oxygen (D, E) or 2% oxygen (D1, E1) for 2–4 serial passages. The number of rat OPCs plated onto either fibronectin or poly-d-lysine obtained at each passage was counted and plotted on a growth curve (F). Data in C and F are representative of at least 3 independent experiments. Scale bars represent 50 µm.$
Fgf 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+fgf-b+protein/Recombinant+human+FGF-b+protein/pmc13596923-78-41-45
Average 94 stars, based on 32 article reviews

fgf 2 - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation"

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation

Journal: ASN NEURO

doi: 10.1080/17590914.2026.2698260

$Mouse OPC growth is accelerated when cultured in medium supplemented with B104CM and FGF-2 and maintained under physiological oxygen conditions. The enriched OPC fraction from neonatal C57Blk/6 mouse mixed glial cell cultures was plated onto fibronectin coated tissue culture plates at 3x10 4 cells/cm 2 and cultured in PDGF-AA+FGF-2 or B104CM + FGF-2 and propagated in 20% oxygen (A, B) or 2% oxygen (A1, B1) for 5 days. The number of cells obtained when grown in B104CM + FGF2 in either 2% or 20% oxygen over 4 serial passages across 35 days is depicted (C). The enriched OPC fractions from mixed mouse glial cell cultures plated onto fibronectin (D) and from mixed rat glial cell cultures plated onto poly-d-lysine (E) and cultured in B104CM + FGF2 in 20% oxygen (D, E) or 2% oxygen (D1, E1) for 2–4 serial passages. The number of rat OPCs plated onto either fibronectin or poly-d-lysine obtained at each passage was counted and plotted on a growth curve (F). Data in C and F are representative of at least 3 independent experiments. Scale bars represent 50 µm.$
Figure Legend Snippet: Mouse OPC growth is accelerated when cultured in medium supplemented with B104CM and FGF-2 and maintained under physiological oxygen conditions. The enriched OPC fraction from neonatal C57Blk/6 mouse mixed glial cell cultures was plated onto fibronectin coated tissue culture plates at 3x10 4 cells/cm 2 and cultured in PDGF-AA+FGF-2 or B104CM + FGF-2 and propagated in 20% oxygen (A, B) or 2% oxygen (A1, B1) for 5 days. The number of cells obtained when grown in B104CM + FGF2 in either 2% or 20% oxygen over 4 serial passages across 35 days is depicted (C). The enriched OPC fractions from mixed mouse glial cell cultures plated onto fibronectin (D) and from mixed rat glial cell cultures plated onto poly-d-lysine (E) and cultured in B104CM + FGF2 in 20% oxygen (D, E) or 2% oxygen (D1, E1) for 2–4 serial passages. The number of rat OPCs plated onto either fibronectin or poly-d-lysine obtained at each passage was counted and plotted on a growth curve (F). Data in C and F are representative of at least 3 independent experiments. Scale bars represent 50 µm.

Techniques Used: Cell Culture, Tissue

OPCs are A2B5± and Ki67± at both low and high density when grown in B104CM ± FGF-2 in 2% oxygen. After the first passage, OPCs were plated onto fibronectin coated chamber slides at 3x10 4 cells/cm 2 (25% confluency) (A) and 6x10 4 cells/cm 2 (50% confluency) (B) and maintained for 4 days in medium supplemented with B104CM + FGF2 in 3 gas incubator with 2% oxygen. Cells were stained for A2B5-green, Olig2-red, and DAPI-blue (A, B) or Olig2-blue and Ki67-red (A1, B1). The number of DAPI+, Olig2+ and Ki67+ cells per field of view (FOV) was quantified (C). Scale bar represents 50 µm. Data represent means ± SEM from three independent experiments.

Figure Legend Snippet: OPCs are A2B5± and Ki67± at both low and high density when grown in B104CM ± FGF-2 in 2% oxygen. After the first passage, OPCs were plated onto fibronectin coated chamber slides at 3x10 4 cells/cm 2 (25% confluency) (A) and 6x10 4 cells/cm 2 (50% confluency) (B) and maintained for 4 days in medium supplemented with B104CM + FGF2 in 3 gas incubator with 2% oxygen. Cells were stained for A2B5-green, Olig2-red, and DAPI-blue (A, B) or Olig2-blue and Ki67-red (A1, B1). The number of DAPI+, Olig2+ and Ki67+ cells per field of view (FOV) was quantified (C). Scale bar represents 50 µm. Data represent means ± SEM from three independent experiments.

Techniques Used: Incubator, Staining

Differential effects of mitogen combinations on the phenotype of cultured OPCs. OPCs were plated onto fibronectin coated chamber slides at 3x10 4 cells/cm 2 in media supplemented with FGF2 or PDGF-AA+FGF-2 and maintained in 2% oxygen. The number of NG2+ cells was counted at 24 and 96 hours after initial plating (A). The ratio of NG2+ cells that were positive for A2B5 or O4 was quantified at 96 hours after plating (B). Data represent means ± SEM from 3 independent experiments. * p < 0.05, *** p < 0.005, **** p < 0.001 by 2-way ANOVA followed by Tukey’s posthoc test. Cells cultured in FGF-2 or PDGF-AA+FGF-2 were stained with antibodies against NG2 (red), PDGFRα (yellow), O4 (green) and DAPI (blue) (C, D). Cells were stained for O4 (green), Olig2 (red), DAPI (blue) (C1, D1) or GFAP (red), A2B5 (green) and DAPI (blue) after 4 days in culture (C2, D2). Scale bar represents 20 µm (C, D) and 200 µm (C1, C2, D1, D2).

Figure Legend Snippet: Differential effects of mitogen combinations on the phenotype of cultured OPCs. OPCs were plated onto fibronectin coated chamber slides at 3x10 4 cells/cm 2 in media supplemented with FGF2 or PDGF-AA+FGF-2 and maintained in 2% oxygen. The number of NG2+ cells was counted at 24 and 96 hours after initial plating (A). The ratio of NG2+ cells that were positive for A2B5 or O4 was quantified at 96 hours after plating (B). Data represent means ± SEM from 3 independent experiments. * p < 0.05, *** p < 0.005, **** p < 0.001 by 2-way ANOVA followed by Tukey’s posthoc test. Cells cultured in FGF-2 or PDGF-AA+FGF-2 were stained with antibodies against NG2 (red), PDGFRα (yellow), O4 (green) and DAPI (blue) (C, D). Cells were stained for O4 (green), Olig2 (red), DAPI (blue) (C1, D1) or GFAP (red), A2B5 (green) and DAPI (blue) after 4 days in culture (C2, D2). Scale bar represents 20 µm (C, D) and 200 µm (C1, C2, D1, D2).

Techniques Used: Cell Culture, Staining

OPCs acquire late progenitor cell markers with time in culture . OPCs were grown in B104CM + FGF-2 in a 3-gas incubator with 2% oxygen, passaged, stained and then analyzed live by flow cytometry at passage 3 (A-A2) and passage 6 (B-B2) using a flow panel comprised of CD133/LeX/NG2/CD140a/O4/GLAST. 50, 000 events were analyzed per group. Forward and side scatter were used to exclude debris and DAPI exclusion was used to define live cells. Blue dots represent isotype controls for background and gating while red dots represent the stained events. Data are representative of two independent experiments.

Figure Legend Snippet: OPCs acquire late progenitor cell markers with time in culture . OPCs were grown in B104CM + FGF-2 in a 3-gas incubator with 2% oxygen, passaged, stained and then analyzed live by flow cytometry at passage 3 (A-A2) and passage 6 (B-B2) using a flow panel comprised of CD133/LeX/NG2/CD140a/O4/GLAST. 50, 000 events were analyzed per group. Forward and side scatter were used to exclude debris and DAPI exclusion was used to define live cells. Blue dots represent isotype controls for background and gating while red dots represent the stained events. Data are representative of two independent experiments.

Techniques Used: Incubator, Staining, Flow Cytometry

OPCs differentiate into MBP+ mature oligodendrocytes in vitro in superphysiological oxygen but most lose their capacity to differentiate after repeated passages. OPCs were grown in B104CM + FGF2 for 3 and 6 passages and then plated onto poly-d-lysine and laminin-coated chamber slides. The growth medium was removed and replaced with B27 growth medium supplemented with 30 nM T3 or 10 ng/mL FGF-2. Cells were maintained in these media for 7 days. Cells were stained for Olig2, MBP and DAPI (Panels A-F,G, K). Cells were stained for O4, Olig2, MBP (Panels H-J and L-N). Scale bar represents 50 µm (A-F) and 100 µm (G,K) and 50 µm (H-J; L-N). Images are representative of three independent experiments.

Figure Legend Snippet: OPCs differentiate into MBP+ mature oligodendrocytes in vitro in superphysiological oxygen but most lose their capacity to differentiate after repeated passages. OPCs were grown in B104CM + FGF2 for 3 and 6 passages and then plated onto poly-d-lysine and laminin-coated chamber slides. The growth medium was removed and replaced with B27 growth medium supplemented with 30 nM T3 or 10 ng/mL FGF-2. Cells were maintained in these media for 7 days. Cells were stained for Olig2, MBP and DAPI (Panels A-F,G, K). Cells were stained for O4, Olig2, MBP (Panels H-J and L-N). Scale bar represents 50 µm (A-F) and 100 µm (G,K) and 50 µm (H-J; L-N). Images are representative of three independent experiments.

Techniques Used: In Vitro, Staining

Recombinant:

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation.
Article Snippet: conditioned medium (B104 CM)(Young & Levison, 1997), or 10 ng/mL recombinant rat platelet-derived growth factor (PDGF-AA) (R&D Systems, Cat# 1055-AA-050) and 10 ng/mL FGF-2 (Peprotech, Cat#100-18C or Alomone Labs, Cat#F-170) with 1 ng/mL heparin sodium (Sigma-Aldrich Cat#H3149). OPCs were plated onto 100 mm tissue culture plates that had been previously coated with 0.5 mg/cm2 fibronectin MW > 440k

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Journal: ASN NEURO

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation

doi: 10.1080/17590914.2026.2698260

Figure Lengend Snippet: Mouse OPC growth is accelerated when cultured in medium supplemented with B104CM and FGF-2 and maintained under physiological oxygen conditions. The enriched OPC fraction from neonatal C57Blk/6 mouse mixed glial cell cultures was plated onto fibronectin coated tissue culture plates at 3x10 4 cells/cm 2 and cultured in PDGF-AA+FGF-2 or B104CM + FGF-2 and propagated in 20% oxygen (A, B) or 2% oxygen (A1, B1) for 5 days. The number of cells obtained when grown in B104CM + FGF2 in either 2% or 20% oxygen over 4 serial passages across 35 days is depicted (C). The enriched OPC fractions from mixed mouse glial cell cultures plated onto fibronectin (D) and from mixed rat glial cell cultures plated onto poly-d-lysine (E) and cultured in B104CM + FGF2 in 20% oxygen (D, E) or 2% oxygen (D1, E1) for 2–4 serial passages. The number of rat OPCs plated onto either fibronectin or poly-d-lysine obtained at each passage was counted and plotted on a growth curve (F). Data in C and F are representative of at least 3 independent experiments. Scale bars represent 50 µm.

Article Snippet: Viable cells were counted and resuspended in proliferation media N2S [B27 growth medium supplemented with 30% B104 neuroblastoma conditioned medium (B104 CM)(Young & Levison, ), or 10 ng/mL recombinant rat platelet-derived growth factor (PDGF-AA) (R&D Systems, Cat# 1055-AA-050) and 10 ng/mL FGF-2 (Peprotech, Cat#100-18C or Alomone Labs, Cat#F-170) with 1 ng/mL heparin sodium (Sigma-Aldrich Cat#H3149).

Techniques: Cell Culture, Tissue

Journal: ASN NEURO

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation

doi: 10.1080/17590914.2026.2698260

Figure Lengend Snippet: OPCs are A2B5± and Ki67± at both low and high density when grown in B104CM ± FGF-2 in 2% oxygen. After the first passage, OPCs were plated onto fibronectin coated chamber slides at 3x10 4 cells/cm 2 (25% confluency) (A) and 6x10 4 cells/cm 2 (50% confluency) (B) and maintained for 4 days in medium supplemented with B104CM + FGF2 in 3 gas incubator with 2% oxygen. Cells were stained for A2B5-green, Olig2-red, and DAPI-blue (A, B) or Olig2-blue and Ki67-red (A1, B1). The number of DAPI+, Olig2+ and Ki67+ cells per field of view (FOV) was quantified (C). Scale bar represents 50 µm. Data represent means ± SEM from three independent experiments.

Techniques: Incubator, Staining

Journal: ASN NEURO

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation

doi: 10.1080/17590914.2026.2698260

Figure Lengend Snippet: Differential effects of mitogen combinations on the phenotype of cultured OPCs. OPCs were plated onto fibronectin coated chamber slides at 3x10 4 cells/cm 2 in media supplemented with FGF2 or PDGF-AA+FGF-2 and maintained in 2% oxygen. The number of NG2+ cells was counted at 24 and 96 hours after initial plating (A). The ratio of NG2+ cells that were positive for A2B5 or O4 was quantified at 96 hours after plating (B). Data represent means ± SEM from 3 independent experiments. * p < 0.05, *** p < 0.005, **** p < 0.001 by 2-way ANOVA followed by Tukey’s posthoc test. Cells cultured in FGF-2 or PDGF-AA+FGF-2 were stained with antibodies against NG2 (red), PDGFRα (yellow), O4 (green) and DAPI (blue) (C, D). Cells were stained for O4 (green), Olig2 (red), DAPI (blue) (C1, D1) or GFAP (red), A2B5 (green) and DAPI (blue) after 4 days in culture (C2, D2). Scale bar represents 20 µm (C, D) and 200 µm (C1, C2, D1, D2).

Techniques: Cell Culture, Staining

Journal: ASN NEURO

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation

doi: 10.1080/17590914.2026.2698260

Figure Lengend Snippet: OPCs acquire late progenitor cell markers with time in culture . OPCs were grown in B104CM + FGF-2 in a 3-gas incubator with 2% oxygen, passaged, stained and then analyzed live by flow cytometry at passage 3 (A-A2) and passage 6 (B-B2) using a flow panel comprised of CD133/LeX/NG2/CD140a/O4/GLAST. 50, 000 events were analyzed per group. Forward and side scatter were used to exclude debris and DAPI exclusion was used to define live cells. Blue dots represent isotype controls for background and gating while red dots represent the stained events. Data are representative of two independent experiments.

Techniques: Incubator, Staining, Flow Cytometry

Journal: ASN NEURO

Article Title: Hyperoxia Inhibits the Growth of Mouse Forebrain Oligodendrocyte Progenitors but Promotes Their Differentiation

doi: 10.1080/17590914.2026.2698260

Figure Lengend Snippet: OPCs differentiate into MBP+ mature oligodendrocytes in vitro in superphysiological oxygen but most lose their capacity to differentiate after repeated passages. OPCs were grown in B104CM + FGF2 for 3 and 6 passages and then plated onto poly-d-lysine and laminin-coated chamber slides. The growth medium was removed and replaced with B27 growth medium supplemented with 30 nM T3 or 10 ng/mL FGF-2. Cells were maintained in these media for 7 days. Cells were stained for Olig2, MBP and DAPI (Panels A-F,G, K). Cells were stained for O4, Olig2, MBP (Panels H-J and L-N). Scale bar represents 50 µm (A-F) and 100 µm (G,K) and 50 µm (H-J; L-N). Images are representative of three independent experiments.

Techniques: In Vitro, Staining

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